Volf P, Grubhoffer L, Hosek P.
ABSTRACT
In mice, the antigen-specific serum antibody response following
exposure to bites of Triatoma infestans nymphs and adults was
determined
using enzyme-linked immunosorbent assay (ELISA). The second and the
third
exposure to T. infestans bites resulted in higher antibody
levels
than during the primary exposure. Antigen-specific antibody development
was faster in mice exposed to bites of nymphs than in mice exposed to
adults;
however, a higher antibody level was found in mice bitten by adult
bugs.
Protein components of T. infestans saliva were characterised
using
electrophoretic and blotting techniques. The immunoblotting patterns
were
similar in mice exposed to insect bites of various developmental
stages.
Antigen-specific serum antibodies reacted only with high molecular mass
components (two double bands of 100 and 120 kDa, and the 80 kDa
antigen).
The 80 kDa component was glycosylated. Affinity to lectin derived from
Pisum sativum revealed the presence of a bi- or triantennary
complex
type of glycan. There were similar glycan structures in the main
glycoprotein
components of T. infestans saliva at 18-35 kDa; however, the
components
did not react with antibodies of mice exposed to insect bites.
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